National Information and Documentation Center (NIDOC), Academy of Scientific Research and TechnologyEgyptian Journal of Microbiology0022-270451120161201Selection of Gamma Radiation Dose for Decontamination of Some Personal Care Products111105310.21608/ejm.2016.1053ENJournal Article20160308IN THIS STUDY, the bacterial evaluation of 74 personal care ….products representing 16 brands revealed that 43 samples representing 10 brands were contaminated with bacteria. The isolated bacterial contaminants from the samples of each brand were prepared in mixed populations to determine the gamma irradiation lethal doses (kGy). The range of the lethal doses was found to be from 10 to 25 kGy. The radiation-resistant bacteria isolates from hair gel brands were <em>Bacillus </em>megaterium<em>, Bacillus</em> brevis and<em> Micrococcus </em>luteus<em>.</em> From hair cream brands, they were <em>Bacillus </em>megaterium<em>, Bacillus subtilis,</em> and <em>Streptococcus </em>mutans<em>.</em> While, from wet wipes brands they were <em>Bacillus </em>megaterium<em>, Bacillus </em>brevis<em>,</em> <em>Micrococcus </em>luteus<em>, Staphylococcus </em>epidermidis and <em>Streptococcus </em>mutans<em>.</em> The radiation-resistant bacteria were selected to study their response to gamma radiation and the D10 values were calculated from the dose response curves and they ranged from 0.65 to 2.2 kGy. The highest lethal doses were applied to the selected contaminated personal care products to ensure the success of the process of decontamination of the tested personal care products using gamma irradiation.https://ejm.journals.ekb.eg/article_1053_d549629ecf5c2273ea57db3f0aa3681a.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and TechnologyEgyptian Journal of Microbiology0022-270451120161231Efficacy of Lactic Acid Bacteria Isolated from Some Fruits and Vegetables1328109110.21608/ejm.2016.1091ENJournal Article20160417THE CURRENT study was conducted to assess the extent of probiotics Lactic acid bacteria(LAB) in some fresh fruits as (peach, pashmala, ,apricot, grape, banana, yellow and red apple, Kiwi, guajava and orange) and some vegetables as (cucumber, tomato and strawberry). Thirty eight (38) isolates were Gram positive and catalase negative, 20 isolates belong to genus<em> Lactobacillus</em> and 18 belong to <em>Lactococcus</em> sp. According to biochemical reactions 13 isolates out of 38 were identified as 5 belong to L. plantarum, 2 isolates to <em>L. brevis</em>, 3 to <em>Pediococcus</em> sp. and 3 to <em>Leuconostoc</em> sp. Antagonistic activity of these isolates against <em>Staphylococcus aureus</em> ATCC6538, <em>Escherichia coli</em> ATCC8739, <em>Pseudomonas</em> <em>aeruginosa</em> ATCC 9027 and <em>Salmonella typhimurium</em> ATCC22 bacteria were assessed. It noticed that some isolates have a negative effect against all the tested isolates, other isolates affected on some tested strains and some isolates affected on all tested strains. In all the cases that have affected the inhibition area not exceeding 5mm. Sensitivity to commercial antibiotics were studied and it noticed that the isolates showed sensitivity to antibiotics varies among the least sensitive isolate, <em>Leuconostoc</em> sp. 26, where it was sensitive to only one antibiotic and the most senior sensitivity was<em> L.plantarum</em> 5 and 27 strains which showed sensitivity to 11 out of 14 antibiotics that were tested . Probiotics characteristics showed that all isolates were sensitive to low stomach pH3 and for 0.3% bile salt, only six isolates were resistant to bile salts 0.3% concentration after 4h; four of Lactobacilli strains (L.plantarum 1, 5 and 38 and L. brevis11 ) and two of Lactococci (Pediococcus sp. 18 and Leuconostoc sp. 21) .https://ejm.journals.ekb.eg/article_1091_b7d21ffce308fe1a5f38737201f427de.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and TechnologyEgyptian Journal of Microbiology0022-270451120161231Isolation and Identification of Zinc Dissolving Bacteria and Their Potential on Growth of Zea mays2943109210.21608/ejm.2016.1092ENAlaa El-Dein Abdelghaffar OmaraAhmedResearcher, soil, water and Environment Research Institute, Agricultural Research Center0000-0001-5622-7501Azza AbdelsalamGhaziResearcher, Soil, Water and Environment Research Institute, Agricultural Research CenterIbrahim Abdel AziamEl-AkhdarResearcher, Soil, Water and Environment Research Institute, Agricultural Research CenterJournal Article20160718A total of twenty-five bacterial isolates obtained from mature compost to evaluate the solubilization potential of insoluble zinc oxide and zinc carbonate. An efficient zinc-solubilizing bacterial isolates (Zn14) were chosen. Thereafter, some of morphological and biochemical characteristics as well as 16S rRNA sequence indicated that the isolate belonging to Enterobacter cloacae. The effect of various carbon and nitrogen sources on the efficiency of Zn solubizilation were investigated under shaking and static conditions. It was found that the selected bacterial strain could solubilize Zn compounds under wide range of nutrient types. <br /> Pot experiment was conducted at Sakha Agricultural Research Station, Sakha, Kafr El-Sheikh, Egypt to study the efficiency of using Enterobacter cloacae as Invivo Zn solubilizer either alone or along with amended zinc compounds (ZnO and ZnCO3) to the soil and its effect on growth parameters of Zea mays plants under sterile and non-sterile soil at the age of 30 and 60 days. <br /> The results indicated that there is a highly significant response to increase Zn uptake in all treatments as compared to control. Also, the treatment with ZnO and E. cloacae was the more efficient in total chlorophyll, dry weight plant-1 and N, P and K percentages compared to control. <br /> Hence, to solve zinc nutrition problem using a biofertilizer, it is obvious that E. cloacae is capable of solubilizing insoluble zinc compounds by inoculation to increase crop growth and saving soil fertility.https://ejm.journals.ekb.eg/article_1092_a1ff1c6d0cc55ad3844bfd8730c984d5.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and TechnologyEgyptian Journal of Microbiology0022-270451120161201Differential Effects of Physical and Chemical Factors on Infectivity of Three Coliphages used as Water Quality Indicator4562109310.21608/ejm.2016.294.1003ENFarag ASamhanNational Research Centre, Dokki, Gizah, Water Pollution Control Dept.,Ahmed AAskoraBotany Department, Faculty of science, Zagazig University, P.O 44519, EgyptSaid AEzzatBotany Department, Faculty of science, Zagazig University, P.O 44519, EgyptEssam IAbu El-NilGreater Cairo potable water company, section of microbiological examination, the 10Th of Ramadan city laboratory, EgyptJournal Article20161019The effect of temperature, pH, UV, petroleum ether (60-80), acetone (99 %), ethyl alcohol (95 %) and chloroform (99 %) on the infectivity of local coliphage isolates was studied. Three E. coli phages (ECP) were isolated from Ismailia canal at Mostorod segment. Ability of local E. coli isolates to serve as a host was evaluated after storage at 4-6º C, 25-28º C and 35-38º C for six months. E. coli isolate number 1 of seven tested served as host for phage reproduction and plaque formation (PF). Phage isolates were identified by plaque size and electron microscopy. ECP 1 and ECP 2 are Podoviridae and ECP3 is a Siphoviridae. The infectivity of coliphages decreased with increasing storage periods and incubation temperature. Exposure of somatic coliphages to acidic or alkaline pH, organic solvents or UV radiation (240 nm) or heating (≥ 60º C) diminished infectivity. Ability of E. coli 1 to serve as a host for somatic coliphages inversely related to its age. No detectable effect was found for lower temperature ≤ 5º C on the infectivity of coliphage isolates during storage periodshttps://ejm.journals.ekb.eg/article_1093_02af0ed3e3756bf71d026652e3296205.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and TechnologyEgyptian Journal of Microbiology0022-270451120161231Production, Characterization and Immobilization of a Fusarium solani Lipase by Chitosan Magnetic Nanoparticles6375109410.21608/ejm.2017.1094ENAhmed M.EL-SayedResearch and Development Center, Misr University for Science and Technology (MUST), Egypt.Wedad E.EwedaAgricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Egypt.Tarek S.EI-TayebAgricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Egypt.Ahmed ZAbdel AzeizCollege of Biotechnology, Misr University for Science and TechnologyJournal Article20161030Lipase producing fungus was isolated and identified as a strain of Fusarium solani based on its 18s rDNA sequencet. The enzyme it produces was purified by diethyl amino ethyl sephadex (DEAE-sephadex) column chromatography. The specific activity of the pure enzyme was 1.98 U/mg protein. The kinetics study showed that Km and Vmax values were 0.63µM and 29.4µM/min/mg protein, respectively. The MW was 95.27 kDa. Effects of pH, incubation temperature and organic solvents on the lipase activity were studied. The maximum enzyme activity was obtained at pH 8.5 and incubation temperature 35°C. Hexane and butanol inhibited enzyme activity by 51% and 72.6 %, respectively, while DMSO stimulated the activity by 47.8%. The lipase was immobilized by fusion to chitosan-coated iron oxide magnetic nanoparticles and cross-linked by glutaraldehyde. The reusability and storage period of the immobilized enzyme showed that the enzyme retained 80% of its activity after 15 reuse cycles and retained 97% of activity after 30 days of storage at 4°C. The immobilized lipase was tested for synthesis of sugars-oleate esters and the ester products were analyzed by liquid chromatography tandem-mass spectrometry (LC/MS/MS). This investigation identified the potential for use of the obtained F. solani lipase in industrial applications that utilize organic solvents or alkaline pH values, such as detergent industry.https://ejm.journals.ekb.eg/article_1094_1e30bfc323a5da33f0120296a04e94a6.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and TechnologyEgyptian Journal of Microbiology0022-270451120161201Biocontrol of Clinical Bacteria Infecting Urinogenital System by Probiotic Bacteria7788109510.21608/ejm.2017.361.1006ENTalaatEl-SayedDepartment of Botany, Faculty of Science, Benha UniversitySoheir SaadAbd El-SalamBotany Department,
Faculty of Science, Benha UniversityGamalEnanBotany Department, Faculty of Science, Zagazig UniversityAhmedShahinMicrobiology Department, Faculty of Medicine, Zagazig UniversityHytham M.AhmedFaculty of Science, Benha UniversityJournal Article20161110Urinogenital infections proved to be one of the most common diseases which mankind can face nowadays. Both men and women can be infected by the urogenital pathogens but the last are considered more susceptible to these injuries due to their physiological nature. The emergence of multi-drug resistance phenomenon made the treatment with such antibiotics is useless. Urinary tract infection (UTI) caused by bacteria is considered one of the most frequent injuries infecting both male and female. The development of multi-drug resistance phenomenon in bacteria leads to the necessity of finding an alternative remedy. Probiotics are suggested as a suitable and appropriate solution. In this study, CFS of Lactobacillus plantarum LPS10 is examined for its inhibitory effect against clinical urogenital bacterial strains and Candida albicans isolated from UTI infections. CFS obtained from this probiotic showed a promising antibacterial and anticandidial results. L. plantarum LPS10 possessed the capability to be used as a probiotic against clinical bacteria isolated from urogenital infections.https://ejm.journals.ekb.eg/article_1095_c855c8936f68c272b97ca5d3787ecac6.pdf