ISM Analysis, Evolution, Human-Type and Validation of UV–spectrophotometric Techniques for Assessment of Sulfadiazine in Bulk and Cream Dose Forms

Document Type : Original Article

Authors

1 Chemistry Department, Faculty of Science, Kufa University, Najaf, Iraq

2 Biology Department, Faculty of Science, Kufa University, Najaf, Iraq

Abstract

A CONVENTIONAL, precise, clear colorimetric technique that is additionally sensitive is evaluated for the appreciation of sulfadiazine medicine as a sharp form, as well as for different sorts of pharmaceutical products with different doses. The technique is premised on the conjugation of Sulfadiazine (SUZ) antibiotic as well as the 3,5Di hydroxy benzoic acid (3,5DHB) agent with utilizing the azo coupling reaction. The (3,5DHB) in the basic media to finally produce a ligand that interacts with Cobalt (II) to generate the strength orange complex color at room temperature. The associated outcomes dye is having a solubility in water as well as flexible additionally predestined colorimetrically with ultimate absorption at (436nm). The calibration graph between the absorbance and the concentration appears that the concentration range from medicine was established with utilizing the Beer’s law was between (0.12 - 18 μg/ml). The eclecticism of the best empirical envelopes is tested. The accuracy additionally the precision for the procedure are calculated by the average recovery amounts (100.122%) as well as the main relative standard deviation amounts (0.762 %) reasonably. That the technique is established on the concentration. The Sensitiveness for the procedure is obtained with molar absorptivity (2.24×104 lcm-1.mol-1). the Sandell sensitivity is tested ( 0.01 μg.cm-2). The analysis facts for The technique. It is agreed upon by the official procedure. The common medicinal additive intervention has been checked. The established technique is analyzed successfully in assessing (SUZ) in various types of pharmaceutical products .
The antibacterial effectiveness capability of three different company sulfadiazine creams toward eight multidrug-resistant bacteria, the findings show that three creams with sulfadiazine had better antibacterial activity on A. Bacterial baumanii isolated as contrasted with another isolated. As for the effectiveness of sulfadiazine, they typically have high antibacterial activity on G-ve bacteria as contrasted to to G +ve bacteria, where Silverdin cream had higher biological efficacy on all bacterial isolates opposed to another creams, and Silverdin’s efficacy equally to pure Sulfadiazine’s antibacterial activity opposed to other two creams.

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