Statistical Optimization of Industrially Potent Streptomyces plicatus MK559563 Xylanase and Α-Amylase Production Utilizing Agricultural Wastes

Document Type : Original Article

Authors

1 Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt

2 Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza, 12622, Egypt

Abstract

Soil isolate Streptomyces plicatus MK559563 succeeded in the biological conversion of agricultural wastes (rice straw RS and potato shells PS) into valuable by-products (reducing sugar and industrial enzymes) by sub merged fermentation technique (SmF). Streptomyces plicatus MK559563 xylanase and α-amylase production were statistically optimized through two designs Plackett-Burman (PB) and Central composite design (CCD). PB design showed that xylanase production by S. plicatus MK559563 was more affected by incubation time, PS weight, and (NH4)2SO4 while α-amylase production was more affected by RS weight, (NH4)2SO4, and CaCl2. CCD improved S. plicatus MK559563 xylanase and α-amylase production by 39.69 and 61.60–fold increase compared to unoptimized media. The optimized media for S. plicatus MK559563 xylanase production composed of g/L: Baker’s yeast (1), (NH4)2SO4 (6.5), KCl (1), ZnSO4 (0.001) with RS (2g/flask) and PS (3.5g/flask) for 7 days of incubation. The optimized media for S. plicatus MK559563 α-amylase production composed of g/L: glucose (10), baker’s yeast (5), (NH4)2SO4 (6.5), CaCl2 (6.5) with RS (3.5g/flask) and PS (1g/flask) for 6 days of incubation. Streptomyces plicatus MK559563 can be used as an intermediate step for bioconversion of agricultural wastes into biofuel.

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