Tetracycline Resistance Genes Prevalence in Enterococcus spp. from Dairy Products in Egypt

Document Type : Original Article

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Botany Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt

Abstract

The frequency and dispersion of tetracycline resistance and virulence traits were investigated in Enterococcus spp. isolated from milk and traditional cheeses in Egypt. Forty-seven presumptive enterococci were isolated, whereas thirty-four Enterococcus spp. isolates were examined for tetracycline antimicrobial sensitivity and antibiotic resistance genes (TET). The findings displayed that Enterococcus spp. isolates were highly resistant to amoxicillin /clavulanate, followed by oxytetracycline, ampicillin, tetracycline, doxytetracycline and ciprofloxacin. The frequency for tetracycline resistance genes( tet K , tetM , tetO and tet S)  was estimated at 85.29% (29/34), 67.65% (23/34),  50 % (17/34) and 29.4 % (10/34) respectively. Six isolates out of seventeen were identified using 16SrRNA as Enterococcus faecalis. Seventeen isolates of Enterococcus spp. and Enterococcus facials isolates were tested for virulence traits. Findings displayed that all the tested Enterococci isolates produced biofilm and gelatinase enzyme except one isolate, which was negative for gelatinase production. On the other hand, all the tested isolates (17) were negative for hemolysin production. It could be concluded that Enterococcus spp. and E. faecalis have continually been recognized as adaptable pathogens, an indicator of faecal pollution in food, and documented for their aptitude to remove genetic material through portable genetic materials and distribute antibiotic resistance amongst extra bacteria. Therefore, faecal contamination of milk and its products must be prevented by applying strict hygienic measures and control of antibiotic use. 

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