Keratinase Production from Chicken Feather by New Egyptian Local Streptomyces Isolates

Document Type : Original Article

Authors

1 Microbial Chemistry Department, Biotechnology Research Institute , National Research Centre, 33 El Behouth st., Dokki P.O. 12622, Giza, Egypt

2 Biochemistry Department , Biotechnology Research Institute , National Research Centre, 33 El Behouth st., Dokki P.O. 12622, Giza, Egypt

Abstract

The main objective of the current research was to isolate new Streptomyces species that has the ability to degrade chicken feathers through keratinase production. Nineteen Egyptian local Streptomyces strains were isolated from soil and tested for their ability to produce keratinase enzyme from chicken feathers. Maximum keratinolytic activity was achieved with a novel Streptomyces isolate strain no 6. that was identified by 16S rDNA method with a given name of Streptomyces sp. NRC FM (Accession no. LC456058). The optimum culture conditions for the highest keratinase production were found to be a pH 9.5, at a temperature of 37˚C and agitation speed of 180 rpm. Interestingly, chicken feathers were completely degraded after 9 days of incubation with the novel strain. Keratinase was precipitated using acetone and its molecular weight was estimated to be 30 and 42 kDa using SDS-PAGE. The results obtained suggest that the isolated novel Streptomyces sp., NRC FM, can be considered as a useful biotechnology tool for valorization of keratin-containing wastes.

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