Application of Langmuir and Freundlich Isotherm Models on Biosorption of Pb2+ by Freez-dried Biomass of Pseudomonas aeruginosa

Document Type : Original Article

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Botany and Microbiology Department, Faculty of Science, Suez University, Suez, Egypt

Abstract

TODAY, the pollution of soil and aquatic environment by lead metal ion results from the discharged industrial waste water represents serious environmental problem. Biosorption is an ecofriendly technology that uses microbial biomasses to bind heavy metals on their surfaces by physicochemical pathways from waste water. Seventy-two bacterial isolates resistant to lead metal ion were recovered from 30 sewage water samples collected from different streets of Faisal district in Suez Governorate, Egypt. Interestingly, the isolate number 2103 was selected among them as the most highly resistant to high concentrations of Pb2+. This isolate was characterized morphologically, biochemically and identified by 16S rRNA gene sequencing as Pseudomonas aeruginosa and deposited in the GenBank database under the accession number KY712434. Studying the effects of contact time and pH on Pb2+ biosorption by the freeze-dried biomass of Pseudomonas aeruginosa 2103 revealed that the maximum biosorption was achieved within 30min at pH 6. The maximum adsorption capacity (qmax) of Pb2+ removal by the freeze-dried biomass of Pseudomonas aeruginosa 2103 was 114.94mg/g. The regression coefficients (R2) were 0.8847 and 0.9751 from the Langmuir and Freundlich isotherm models, respectively, so the biosorption of Pb2+ onto the biomass of Pseudomonas aeruginosa 2103 has been found to fits Freundlich isotherm model better than Langmuir isotherm model.

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